姓名：吴克良
邮箱：wukeliang_527@163.com
个人主页：
https://webvpn.sdu.edu.cn/http-8080/77726476706e69737468656265737421a1a70fcd696226092b/system/caslogin.jsp
个人简介/Personal resume
吴克良，山东大学教授/博导，国家级青年人才，山东省级青年专家。现任山东大学附属生殖医院院长助理，辅助生殖实验室主任，国家辅助生殖技术管理专家库成员，中华医学会生殖医学分会生殖医学实验室学组委员，中国性学会生女性生殖医学分会副主任委员。
长期从事人类辅助生殖技术及早期胚胎发育的相关临床和基础研究，先后主持国家重点研发计划（课题组长）、国家自然科学基金等国家级课题6项；以第一或通讯作者身份在Nature、Cell、Cell Research、Nat Commun、Nat Struct Mol Biol、Cell Discovery、Natl Sci Rev等杂志发表SCI论文近30篇；获教育部科技进步一等奖、山东省青年科技奖；获发明专利10余项，转化4项，直接经济价值超过2500/万年。
研究领域/Research direction
本课题组研究---人类胚胎发育相关研究包括人类早期胚胎发育的调控机制（表观遗传、印记基因）、人类早期胚胎发育异常病理机制和辅助生殖新技术的临床应用。
主要研究课题：1. 人类早期胚胎发育的表观遗传调控网络研究（染色体开放性、3D结构、组蛋白修饰、印记基因等）
2. 人孤雌、孤雄单倍体胚胎及干细胞的研究
3. 线粒体遗传疾病的治疗手段——胞浆置换（全球首创：前原核移植、第一极体移植、第二极体移植）
招生信息/Enrollment information
招生学科（一级和二级学科）：基础医学、基础科学
学科方向：细胞生物学/生物学
荣誉奖励/Honors & Awards
国家级青年人才
山东省青年专家
山东大学杰出中青年学者第一层次
齐鲁青年学者
教育部科技进步一等奖
中华医学青年科技奖（第2位）
山东省青年科技奖（第1位）
山东省科技创新成果二等奖（第4位）
代表性论文/Representative publications
1.Xuepeng Chen△, Yuwen Ke△, Keliang Wu(Co-first authors) △, Han Zhao△, Yaoyu Sun, Lei Gao, Zhenbo Liu, Jingye Zhang, Wenrong Tao, Zhenzhen Hou, Hui Liu, Jiang Liu* & Zi-Jiang Chen*. Key role for CTCF in establishing chromatin structure in human embryo. Nature. 2019; 576(7786): 306-310. (IF: 69.504)
2.Lei Gao△, Keliang Wu(Co-first authors) △, Zhenbo Liu, Xuelong Yao, Shenli Yuan, Wenrong Tao, Lizhi Yi, Guanling Yu, Zhenzhen Hou, Dongdong Fan, Yong Tian, Han Zhao, Jianqiao Liu*, Zi-Jiang Chen*, Jiang Liu*. Chromatin Accessibility Landscape in Human Early Embryos and its Association with Evolution. Cell. 2018 Mar 22;173(1):248-259.e15. (IF: 66.850)
3.Shenli Yuan△, Jianhong Zhan△, Jingye Zhang△, Zhenbo Liu, Zhenzhen Hou, Chuanxin Zhang, Lizhi Yi, Lei Gao*, Han Zhao*, Zi-Jiang Chen*, Jiang Liu*, Keliang Wu*. Human zygotic genome activation is initiated from paternal genome. Cell Discovery. 2023 Jan 31;9(1):13.（IF:38.272)
4.Keliang Wu△; Dongdong Fan△; Han Zhao; Zhenbo Liu; Zhenzhen Hou; Wenrong Tao; Guanling Yu; Shenli Yuan; Xiaoxiao Zhu; Mengyao Kang; Yong Tian; Zi-Jiang Chen*; Jiang Liu*; Lei Gao* ; Dynamics of histone acetylation during human early embryogenesis., Cell Discovery., 2023, 9(1):29
5.Yusheng Liu△, Han Zhao△, Fanghong Shao△, Yiwei Zhang△, Hu Nie, Jingye Zhang , Cheng Li, ZhenzhenHou, Zi-Jiang Chen*, JiaqiangWang*, Bing Zhou*, Keliang Wu*, Falong Lu*.Remodeling of maternal mRNA through poly(A) tail orchestrates human oocyte-to-embryo transition.Nature Structural & Molecular Biology.2023 Jan16 doi: 10.1038/s41594-022-00908-2.(IF: 18.361)
6.Honghui Zhang△, Tailai Chen△, Keliang Wu(Co-first authors)△, Zhenzhen Hou△, Shigang Zhao, Chuanxin Zhang, Yuan Gao, Ming Gao, Zi-Jiang Chen* & Han Zhao*. Dominant mutations in CHK1 cause pronuclear fusion failure and zygote arrest that can be rescued by CHK1 inhibitor. Cell Res. 2021 Jul;31(7):814-817. (IF: 46.297)
7.Xiaoyu Merlin Zhang△, Keliang Wu(Co-first authors)△, Yuxuan Zheng△, Han Zhao△, Junpeng Gao, Zhenzhen Hou, Meiling Zhang, Jiaoyang Liao, Jingye Zhang, Yuan Gao, Yuanyuan Li, Lin Li, Fuchou Tang*, Zi-Jiang Chen*, Jinsong Li*.In vitro expansion of human sperm through nuclear transfer. Cell Res. 2020,30(4):356-359. (IF: 46.297)
8.Keliang Wu(Co-first authors) △, Tailai Chen△, Sexin Huang, Cuiqing Zhong, Junhao Yan, Xiaoyu Zhang, Jinsong Li, Yuan Gao*, Han Zhao*, Zi-Jiang Chen*. Mitochondrial replacement by pre-pronuclear transfer in human embryos. Cell Research. 2017 Jun; 27(6):834-837. (IF: 46.297)
9.Keliang Wu(Co-first authors) △, Cuiqing Zhong△, Tailai Chen△, Xiaoyu Zhang, Wenrong Tao, Jingye Zhang, Hongchang Li, Han Zhao*, Jinsong Li* and Zi-Jiang Chen*.Polar bodies are efficient donors for reconstruction of human embryos for potential mitochondrial replacement therapy. Cell Res. 2017 Aug; 27(8):1069-1072. (IF: 46.297)
10.Cuiqing Zhong, Meiling Zhang, Qi Yin, Han Zhao, Yang Wang, Sexin Huang, Wenrong Tao, Keliang Wu*, Zi-Jiang Chen*, and Jinsong Li*. Generation of human haploid embryonic stem cells from parthenogenetic embryos obtained by microsurgical removal of male pronucleus. Cell Res. 2016 Jun; 26(6):743-6. (IF: 46.297)
